Immune monitoring simply refers to the observation of temporal changes in the major cell population and some intracellular markers. In our "basic immune monitoring" service, the major cell types include B cell, NK cell, Monocyte, DC, and T cells. However, a few more surface and intracellular markers can be used to further identify and monitor immune cell types, such as cytotoxic T cell and T helper cells.
Typically, customized panels of ~ 25 markers are used in our immune monitoring service. Manual gating strategies are used to quantify immune cell populations and visualized with t-SNE or UMAP dimensionality reduction techniques.
Number of markers : ~ 25
Number of cell types and their subsets : 5 - 10
Dimensionality reduction & 2D-visualization : t-SNE, UMAP
Manual gating : Included
Quantification of immune cell populations : included
Our service workflow is here !!