scRNAseq

Assessing immune cell type heterogeneity and transcriptomic profiling of expressed genes from significant lymphocytes at a single level provides a more in-depth understanding of the complexity characteristic of immune cell types. Furthermore, these analytical approaches provide immune cell subsets and differently expressed genes (DEGs) information responsible for pathogenic inflammatory cytokines via the relevant molecular pathways. Traditional RNAseq analysis has limitations in revealing the complexity of target immune cell subsets and their transcriptomic changes. To overcome these limitations, single cell RNA sequencing (scRNAseq) analysis has proven to be a critical technological approach for visualizing in-depth rare immune subsets with unprecedented detail, allowing basic scientific facts to be discovered

Analytical Approaches

• Comprehensive scRNAseq analysis for Human, Mice and Rat PBMC samples

• Comprehensive scRNAseq analysis for Human Cancer Lines

• Comprehensive scRNAseq analysis for various Human Pathological Diseases

• Single Cell Technology - 10X Genomics – Single Cell Captue

• BCR/TCR Repertoire Analysis -Determining Immunological Status for Vaccine Development

• Cellular Indexing of Transcriptomes and Epitopes (CITE-seq) Sequencing Analysis

Advantages of scRNAseq Analysis

• Identification of Heterogeneous Immune Leukocytes by Reference Marker Genes

• Immune Subsets Identification

• High-dimensional data visualization of scRNAseq analysis

• tSNE, UMAP visualization

• Cellular Comparison (Between the Cell Types, With in Cell Types)

• DEGs analysis of Immune Subsets

• Network Analysis

• Molecular pathway approaches